NSET – Non-Surgical Embryo Transfer Device
NSET mediated embryo transfer can replace oviduct surgery for the production of transgenic mice via microinjection.
The NSET Device can be used to transfer blastocysts with gene-targeted R1 ES cells.
The NSET Device can be used to transfer cryopreserved embryos.
NSET assists researchers in meeting 2 of Russell & Burch’s 3R’s:
Reduction:
NSET has the potential to reduce the number of animals as the recipient mother can be re-used
Refinement:
NSET, as a refinement helps enhance animal welfare by minimising the potential pain, suffering
and distress of surgery
NSET References
Green et al.Biotechniques. 2009 Nov; 47(5):919-24
NSET UK Published Poster. By Cancer Research UK
NSET Device Cost Analysis
| Surgical Procedures | NSET Procedures | |
|---|---|---|
| Minutes Per Procedure* | 60 | 2 |
| Labour & Overhead/Minute** | 60 x £1.04 | 2x £1.04 |
| Labour & Overhead Cost/Procedure | £62.40 | £2.08 |
| Consumable Cost of NSET*** | N/A | 27.00 |
| Total Cost of Procedure | £62.40 | £29.08 |
| NSET Price Reduction | 53% |
*** Cost analysis does not include reduction of surgical consumables.