NSET Device
Non-Surgical Embryo Transfer Device
NSET
NSET – Non-Surgical Embryo Transfer Device
The NSET device revolutionises embryo transfer in pseudopregnant mice, making it ideal for producing transgenic or knock-out mice without surgery. The NSET device attaches to a P-2 pipette to allow for the transcervical transfer of embryos. NSET technology is as effective as current embryo surgical procedures, but requires significantly less time to master than traditional surgery.
NSET Device Uses:
- Embryo transfer after DNA microinjection
- Transfer of ES cell chimeric blastocysts
- Transfer of cryopreserved embryos
- Transfer of cells after in vitro fertilisation
- Embryo transfer for rederivation
Advantages of using the NSET:
- Reduces costs of embryo transfer up to 75%
- Eliminates the pain and distress of surgery
- No anaesthesia required
- Eliminates need for post-surgical monitoring of animals
- Reduces regulatory burden by eliminating need to justify survival surgery
- Eliminates surgical instruments and time consuming pre andpost surgical processes
- Greatly reduces time required to become proficient in embryo transfer
NSET Device Cost Analysis
| Surgical Procedures | NSET Procedures | |
|---|---|---|
| Minutes Per Procedure* | 60 | 2 |
| Labour & Overhead/Minute** | 60 x £1.04 | 2x £1.04 |
| Labour & Overhead Cost/Procedure | £62.40 | £2.08 |
| Consumable Cost of NSET*** | N/A | 36.00 |
| Total Cost of Procedure | £62.40 | £38.08 |
| NSET Price Reduction | 39% |
*** Cost analysis does not include reduction of surgical consumables.
Non-Surgical Embryo Transfer Device (NSET Device) from reveal on Vimeo
The Non-Surgical Embryo Transfer (NSET) device, which was developed at the University of Kentucky, represents a significant advance in the transfer of mouse embryos
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